Abcam, ab265453, Human PCOLCE2 (PCPE-2) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PCOLCE2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 1 and 16 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 1 and 16 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PCOLCE2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PCPE-2 also known as procollagen C-endopeptidase enhancer 2 enhances the activity of procollagen C-proteinase. It weighs approximately 42 kDa. PCPE-2 is expressed in various tissues including liver and kidney. This protein influences collagen biosynthesis by binding to substrates and increasing enzymatic activity.
Biological function summary
PCPE-2 assists in the maturation of type I II and III procollagens. It does not form a part of any larger complex but it increases the cleavage efficiency of the C-terminal propeptide from procollagens. By promoting collagen fibril formation PCPE-2 plays a role in tissue organization and structure.
Pathways
The role of PCPE-2 in collagen biosynthesis connects it to the extracellular matrix organization pathway. Additionally this protein interacts with enzymes such as bone morphogenetic protein 1 (BMP1) which are important for collagen maturation. These interactions facilitate the assembly and structure of the extracellular matrix.
PCPE-2's involvement in collagen maturation links it to fibrosis a condition characterized by excessive collagen deposition. It also relates to osteogenesis imperfecta a disorder affecting bone strength. In these conditions PCPE-2 works alongside proteins like BMP1 influencing collagen processing and accumulation thereby affecting tissue characteristics.