Abcam, ab265487, Human SLC22A5 (Solute carrier family 22 member 5) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SLC22A5 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC22A5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Solute carrier family 22 member 5 (SLC22A5) also known as OCTN2 is a transporter protein with a molecular mass of approximately 62 kDa. It facilitates the uptake of organic cations and carnitine a type of amino acid derivative into cells. SLC22A5 is expressed widely in tissues with notable presence in skeletal muscle heart kidney intestine and placenta where it plays a significant role in cellular nutrition and detoxification processes.
Biological function summary
SLC22A5 functions to maintain carnitine homeostasis in the body. Carnitine is essential for the transport of long-chain fatty acids into the mitochondria for β-oxidation therefore providing cellular energy. SLC22A5 is a component of the carnitine transport system and interacts directly with other transporters and enzymes involved in the carnitine pathway. It is responsible for mediating the uptake and efflux of L-carnitine and acyl-carnitines across the cell membrane.
Pathways
The involvement of SLC22A5 in fatty acid metabolism is significant. It importantly participates in the carnitine shuttle allowing fatty acids to enter the mitochondria which is integral to energy production via fatty acid β-oxidation. SLC22A5 is linked with the long-chain fatty-acid-CoA ligase proteins which activate fatty acids prior to mitochondrial uptake. The interaction with these proteins ensures efficient energy production in response to cellular demands.
Defective SLC22A5 function is associated with systemic primary carnitine deficiency (CDSP) a disorder characterized by impaired cellular energy metabolism. This deficiency results from a loss of function mutations in the SLC22A5 gene leading to deficient carnitine transport and accumulation of fatty acids in tissues. Additionally SLC22A5 interactions with proteins involved in energy metabolism may play a role in metabolic syndromes as energy imbalance can contribute to the clinical manifestations of these disorders.