Abcam, ab265504, Human TAF11 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TAF11 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TAF11, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TAF11 also known as Transcription Initiation Factor TFIID Subunit 11 is a component of the transcription factor TFIID complex which is essential for RNA polymerase II-mediated transcription initiation. This protein has a molecular mass of approximately 21 kDa. TAF11 is expressed in various tissues with a strong expression in the liver and to a lesser extent in the heart and skeletal muscle indicating its widespread role in gene regulation across different organs.
Biological function summary
TAF11 functions as a core component of the TFIID complex which consists of the TATA-binding protein (TBP) and multiple TBP-associated factors (TAFs). The complex plays an important role in the regulation of gene expression by assisting in the formation of the transcription preinitiation complex at gene promoters. By interacting with other subunits within the TFIID complex TAF11 helps in the precise transcription of genes supporting cellular processes.
Pathways
TAF11 plays a central role in the transcription regulation pathway and is also involved in the RNA polymerase II transcription initiation pathway. It interacts with multiple factors in these pathways including TAF6 and TAF9 which are other TAF proteins that collaborate in stabilizing the transcription preinitiation complex. These interactions support its involvement in the regulation of both basal and activated transcription influencing gene expression patterns.
Some studies suggest TAF11 association with cancer and developmental disorders. Alterations in the transcription regulation pathway where TAF11 is a component may contribute to oncogenesis making it an area of interest for cancer research. Additionally dysregulation of associated proteins like TAF13 could influence developmental processes linking TAF11 to potential developmental disorders though the precise mechanisms remain to be fully understood.