Abcam, ab265573, Human SLC50A1 (RAG1AP1) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SLC50A1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1 and 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 1 bp insertion in exon 1 and 2 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC50A1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RAG1AP1 also known as RAG1-activating protein 1 or RAG1/DCAF1-interacting protein interacts with various components at the mechanical level to regulate gene expression. The protein has a molecular mass of approximately 50 kDa. RAG1AP1 expression is seen in several tissues with higher levels observed predominantly in immune-related cells suggesting a specific function in those areas.
Biological function summary
The activity of RAG1AP1 involves modulating the immune system through its role in gene regulation. It participates in a complex interaction with proteins involved in the DNA damage response which is important for maintaining genomic stability. RAG1AP1 impacts the activation of genes necessary for immunological processes influencing how cells respond to immune challenges.
Pathways
RAG1AP1 plays a role in adaptive immunity and DNA repair pathways. The protein integrates into the broader context of the V(D)J recombination pathway directly interacting with proteins like RAG1 and RAG2 that are involved in recombination events essential for antibody diversity. Additionally RAG1AP1 relates to ubiquitination pathways that affect protein degradation and stability including factors like DCAF1.
Mutations or dysregulation in RAG1AP1 link with immunodeficiency disorders where patients may have compromised immunity due to faulty V(D)J recombination. Moreover RAG1AP1 associations with DNA repair processes connect it to certain cancer types where a malfunction in these pathways can lead to genomic instability. Its interaction with RAG1 in particular highlights its importance in maintaining proper immune function and genomic integrity.