Abcam, ab265639, Human TMEM69 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TMEM69 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 3,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMEM69, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The TMEM69 protein also referred to as Transmembrane Protein 69 has a mass of approximately 31 kDa. It acts as an integral membrane protein involved in lipid signaling and cellular communication. Expression of TMEM69 occurs in various tissues with notable presence in the brain and heart. Structurally its transmembrane domains facilitate interaction with other membrane proteins enabling effective signal transmission across the cell membrane.
Biological function summary
The functions associated with TMEM69 contribute to cellular signaling processes and membrane trafficking. The protein does not form part of larger protein complexes itself but interacts with several membrane-associated proteins for diverse cellular roles. Its activity impacts cellular homeostasis influencing both signal transduction pathways and the maintenance of cellular machinery.
Pathways
TMEM69 impacts both the PI3K/AKT signaling and ERK/MAPK pathways influencing cell growth and survival. In the PI3K/AKT pathway it enhances kinase activity contributing to cell survival and proliferation. Within the ERK/MAPK pathway TMEM69 modulates protein interactions leading to changes in cell differentiation. Proteins like AKT and ERK1/2 interact closely with TMEM69 in these pathways highlighting its involvement in critical cellular processes.
TMEM69 exhibits connections to neurodegenerative diseases and cardiovascular disorders. Altered expression of this protein in brain tissues links it to Alzheimer's disease where it may affect neural cell survival through the PI3K/AKT pathway. In the context of heart disease TMEM69 interacts with proteins such as PI3K suggesting a role in cardiac cell response to stress. These links mark TMEM69 as a protein of interest for disease studies related to neurological and cardiac health.