Abcam, ab265671, Human RNF149 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RNF149 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RNF149, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNF149 also known as RING Finger Protein 149 is an E3 ubiquitin-protein ligase which means it catalyzes the transfer of ubiquitin from an E2 ubiquitin-conjugating enzyme to a substrate protein tagging it for degradation. The mass of RNF149 is approximately 49 kDa. It can be found expressed in various tissues with notable presence in the liver and kidney. This protein contains a RING-type zinc finger domain which is critical for its ligase activity and substrate specificity.
Biological function summary
RNF149 participates in protein quality control by mediating the ubiquitination of proteins that require degradation therefore maintaining protein homeostasis. It acts independently and is not part of a larger protein complex. By tagging damaged or misfolded proteins for degradation RNF149 contributes to the cellular stress response ensuring that cells respond to potential stressors effectively and efficiently.
Pathways
The involvement of RNF149 in the ubiquitin-proteasome system places it within the protein degradation pathway which is essential for regulating protein turnover and cellular function. It interacts with several other proteins including ubiquitin-conjugating enzyme E2s in this pathway. RNF149 may also intersect with apoptosis pathways due to its role in controlling the degradation of apoptotic regulators linking it to proteins involved in cell death processes.
RNF149 shows a connection to certain cancers and neurodegenerative diseases. Abnormal expression or mutations in RNF149 can relate to the deregulation of protein turnover contributing to the development of hepatocellular carcinoma. It is also connected to neurodegenerative disorders due to its role in degrading damaged proteins with connections to proteins like p53 in cancer pathways and tau proteins in neurological contexts.