Abcam, ab265689, Human MTMR14 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MTMR14 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 1 and 2 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MTMR14, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MTMR14 also known as Jumpy or KIAA0788 is a member of the myotubularin-related protein family. This protein functions as a phosphatase where it dephosphorylates phosphatidylinositol 3-phosphate (PI3P) and phosphatidylinositol 35-bisphosphate (PI(35)P2). MTMR14 has a molecular mass of approximately 87 kDa. Researchers find its expression in various tissues with substantial levels in muscle and brain tissue.
Biological function summary
MTMR14 plays a role in maintaining intracellular membrane dynamics. It forms part of a complex with other myotubularins which regulate phosphoinositide levels. By modulating these lipids MTMR14 helps control endocytosis autophagy and membrane trafficking. This regulation is essential for normal cellular function and homeostasis.
Pathways
MTMR14 is involved in the PI3K-Akt signaling pathway and the phosphatidylinositol signaling system. It interacts with proteins like Akt and mTOR influencing cellular processes such as growth and metabolism. These pathways are important for cell survival growth and differentiation demonstrating MTMR14's role in comprehensive cellular functions.
MTMR14's dysfunction links to conditions like centronuclear myopathy and Charcot-Marie-Tooth disease. These disorders result from mutations impacting MTMR14's phosphatase activity. MTMR14 interacts with other proteins such as dynamin 2 in these conditions highlighting its relevance to disease pathogenesis and potential as a therapeutic target.