Abcam, ab265691, Human SMEK2 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PPP4R3B KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PPP4R3B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SMEK2 also known as protein phosphatase 4 regulatory subunit 3B (PPP4R3B) is a regulatory subunit of the protein phosphatase 4 (PP4) complex. SMEK2 exhibits a molecular mass of about 106 kDa. It is distributed in various tissues with notable expression in the brain and skeletal muscle. As part of the PP4 complex its primary role is to guide and regulate the specific functions of the catalytic subunits of the complex.
Biological function summary
SMEK2 regulates cellular processes by modulating phosphatase activity. It acts as part of the PP4 complex which includes the PP4 catalytic subunit and other regulatory subunits. This complex plays a role in diverse biological functions such as cellular response to DNA damage and cell division. By affecting dephosphorylation events SMEK2 influences cellular signaling allowing for precise cellular responses under varying conditions.
Pathways
SMEK2 significantly impacts the DNA damage response and cell cycle regulation pathways. It interacts closely with proteins such as PP4C the catalytic subunit of the PP4 complex and has connections to key checkpoint regulators in these pathways. Such interactions ensure accurate DNA repair and proper progression through cell cycle phases. Changes in SMEK2's functionality or expression can disrupt these pathways leading to improper cellular responses.
Altered SMEK2 activity or expression has been associated with cancer and neurodegenerative diseases. In cancer SMEK2 along with PP4C influences tumor suppressor pathways by regulating processes like DNA damage repair. Disruption in these interactions can lead to uncontrolled cell proliferation. Additionally abnormalities in SMEK2 have been linked to Alzheimer's disease possibly due to its involvement in neuronal signaling pathways and response to cellular stress.