Abcam, ab265705, Human NDC1 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
NDC1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 2 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 2 and 2 bp deletion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NDC1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NDC1 also known as Nucleoporin NDC1 is an important component of the nuclear pore complex. It is a highly conserved protein weighing approximately 75 kDa. NDC1 localizes to the nuclear envelope. This protein integrates into the nuclear pore complex which facilitates the transport of molecules across the nuclear envelope. NDC1's structural role is significant for maintaining nuclear pore complex integrity during cell division in eukaryotic cells.
Biological function summary
NDC1 interacts with other nucleoporins to form a functional core within the nuclear pore complex. It plays a critical role in regulating the trafficking of macromolecules between the cytoplasm and nucleus. As part of a dynamic assembly NDC1 ensures that nuclear-cytoplasmic transport processes remain efficient. This protein's interaction with other nucleoporins like NUP53 and NUP93 suggests a scaffold function in pore architecture and assembly.
Pathways
NDC1 integrates into the nucleocytoplasmic transport pathway and is essential in cell cycle regulation. This pathway is important for maintaining cellular homeostasis as it manages the exchange of proteins and RNA. NDC1 works alongside proteins such as NUP205 and Gle1 to facilitate effective transport mechanisms. By supporting such pivotal processes NDC1 helps in ensuring proper cellular functionality and division.
NDC1's dysfunction associates with conditions such as cancer and developmental abnormalities. Abnormalities in NDC1 expression or modification can lead to disruptions in nuclear transport contributing to tumorigenesis. Such imbalances might affect its interaction with other proteins like p53 a known tumor suppressor. Understanding NDC1's role could provide insights into targeted therapies for these conditions.