Abcam, ab265727, Human LDB1 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
LDB1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LDB1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LDB1 also known as LIM Domain Binding 1 serves an important role in transcriptional regulation. This protein with a mass of approximately 42 kDa frequently expresses in tissues such as the brain heart and spleen. LDB1 participates in forming multiprotein complexes through its interaction with LIM homeodomain proteins engaging with various co-regulators to influence gene expression.
Biological function summary
LIM Domain Binding 1 plays an important role in developmental processes and cell differentiation. It combines with other proteins to form a LIM complex that acts as a DNA-binding transcriptional regulator. The complex binds to enhancer elements and facilitates the activation or repression of genes critical for the development of the nervous system and cardiovascular system.
Pathways
LIM Domain Binding 1 integrates into the Wnt signaling pathway and the TGF-beta pathway. It interacts with proteins such as TCF/LEF and Smad serving as a cofactor that modulates transcriptional responses and influences cellular outcomes. By participating in these pathways LDB1 helps regulate cell fate decisions ensuring appropriate cellular differentiation and proliferation responses.
LIM Domain Binding 1 holds relevance to leukemia and Wilms' tumor. Its dysregulation connects with disproportionate expression of key developmental genes contributing to pathogenesis. LDB1's interaction with transcription factors like SCL/TAL1 highlights its contribution to the onset of these diseases by modulating gene expression patterns associated with these cancers.