Abcam, ab265733, Human SLC30A7 (ZnT-7) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SLC30A7 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 4 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 4 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC30A7, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZnT-7 also known as SLC30A7 is a zinc transporter protein responsible for decreasing cytosolic zinc levels by transporting zinc ions into the Golgi apparatus and trans-Golgi network. The molecular weight of ZnT-7 is approximately 45 kDa. It is expressed in many tissues with high levels found in the liver kidney and heart. The protein is localized mainly in the intracellular membranes playing a role in intracellular zinc homeostasis.
Biological function summary
ZnT-7 participates in the regulation of cellular zinc balance and is important for maintaining optimal enzyme activity and protein function. Although it does not form part of a larger complex itself it interacts with other zinc transporters to help coordinate zinc distribution within cells. This management of zinc levels is important for preventing zinc toxicity and supporting normal cellular activities which are dependent on zinc as a cofactor.
Pathways
ZnT-7 is involved in the zinc homeostasis pathway controlling the intracellular sequestration and distribution of zinc. It works alongside other zinc transporters such as ZnT-1 and ZIP family members to maintain appropriate intracellular zinc levels. ZnT-7 also affects the insulin signaling pathway as zinc ions are modulators of insulin storage and secretion in pancreatic beta cells.
Alterations in ZnT-7 function or expression have been linked to type 2 diabetes and prostate cancer. Disruption of zinc homeostasis due to ZnT-7 malfunction can contribute to metabolic dysfunctions and tumor development. The protein's interaction with other zinc transporters including ZnT-10 highlights its role in these conditions emphasizing the need for proper regulation of zinc levels in disease prevention and management.