Abcam, ab265808, Human MZT1 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MZT1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 55 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 55 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MZT1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MZT1 also known as Mitotic Spindle Organizing Protein 1 functions in the assembly and organization of the mitotic spindle during cell division. MZT1 has a molecular mass of approximately 10 kDa. It is expressed in various tissues particularly in rapidly dividing cells due to its role in mitosis. MZT1 localizes mainly to the centrosomes which are critical for spindle formation and function during mitosis.
Biological function summary
The small MZT1 protein integrates into the core structure of the mitotic apparatus by being part of the γ-tubulin ring complex (γ-TuRC). This complex is important for microtubule nucleation at the centrosome. By ensuring proper organization of the microtubules MZT1 supports accurate chromosomal segregation and cell division. These functions make MZT1 significant in maintaining genomic stability during cell replication.
Pathways
Ensuring precise spindle organization and function involves MZT1 in the regulation of the cell cycle and mitotic checkpoint pathways. It connects with proteins such as γ-tubulin and other spindle-associated proteins to facilitate microtubule dynamics. These interactions place MZT1 firmly within pathways that oversee correct mitotic progression and check for errors during cell division preventing chromosomal instability.
Improper function or expression of MZT1 has connections with cancer and some chromosomal instability syndromes. Its association with key mitotic processes means that disruptions can lead to unregulated cell proliferation a hallmark of cancer. Altered interactions between MZT1 and the γ-tubulin complex can contribute to tumorigenesis highlighting its potential as a target for therapeutic approaches.