Abcam, ab265812, Human TNKS1BP1 (Tankyrase binding protein 1) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TNKS1BP1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TNKS1BP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Tankyrase binding protein 1 also known as TAB182 or TNKS1BP1 interacts directly with tankyrase enzymes. It has a molecular mass of approximately 182 kDa. The protein mainly localizes in the cytoplasm and nucleus. Its interaction with tankyrase a poly(ADP-ribose) polymerase modulates various cell processes. This binding suggests TAB182 has important roles in managing protein stability and cellular localization reactions.
Biological function summary
TAB182 participates heavily in cellular regulation activities. It forms complexes with tankyrase contributing to the control of telomere length and maintenance. By engaging with numerous other proteins TAB182 influences functions like Wnt signaling and Axin degradation. It interacts with tankyrase to impact cellular structures like centrosomes hinting at a broad regulatory role in cell cycle regulation.
Pathways
TAB182's interaction with tankyrase places it within notable cellular pathways. It plays a role in the Wnt/β-catenin signaling pathway which regulates gene transcription and cellular differentiation. In this pathway TAB182 works closely with Axin and the destruction complex. Furthermore TAB182 is linked to the telomere elongation pathway collaborating with TRF1 to regulate telomere maintenance inheriting tankyrase association roles.
TAB182 connection implicates it in cancer and metabolic disorders. In cancer TAB182 dysregulation might alter telomere maintenance facilitating oncogenesis in conjunction with proteins like TRF1. Regarding metabolic disorders disruptions in Wnt signaling pathways involving TAB182 and Axin could contribute to obesity or type 2 diabetes as these pathways regulate adipogenesis and insulin sensitivity. Understanding TAB182's role helps uncover potential therapeutic targets for these diseases.