Abcam, ab265870, Human TM9SF4 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TM9SF4 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TM9SF4, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TM9SF4 also known as Transmembrane 9 Superfamily Member 4 is a multi-pass membrane protein with an approximate molecular mass of 70 kDa. It is localized in the Golgi apparatus and endosomes showing a notable expression in immune-related cells such as macrophages and dendritic cells. TM9SF4 is involved in the transport and sorting of proteins within cellular compartments playing an important role in maintaining intracellular pH homeostasis by modulating ion exchange across membranes.
Biological function summary
As part of a larger protein family TM9SF4 is involved in the regulation of cellular processes like endocytosis and exocytosis. It functions as a regulator of phagocytosis a process critical for immune response and cell clearance. Through its action in cellular compartments it contributes to antigen presentation thereby influencing cellular immune responses. Its involvement in these pathways highlights its significance in both normal cellular functions and immune system regulation.
Pathways
TM9SF4 influences processes associated with the endocytic pathway and vesicular trafficking. It interacts within cellular networks involving SNARE proteins responsible for vesicle fusion highlighting its role in transport pathways. TM9SF4 also associates with V-ATPase complexes affecting acidic environments necessary for hydrolytic enzymes in endosomes. Through these connections TM9SF4 helps sustain cellular dynamics and integrity.
TM9SF4 links to immune-related conditions particularly chronic inflammatory diseases. It affects macrophage function and inflammation making it relevant in disorders such as rheumatoid arthritis. Additionally TM9SF4 alterations associate with tumor microenvironments influencing cancer progression. Through its modulation of phagocytic functions abnormalities in TM9SF4 activity can alter interactions with proteins like TNF-alpha which are important in inflammation and cancer-related pathways.