Abcam, ab265880, Human MIOS knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MIOS KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 4,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MIOS, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MIOS also known as mitotic spindle organizing protein or GATOR2 subunit is a protein that plays an important role in cell cycle progression. It has a mass of approximately 67 kDa. MIOS is expressed in various tissues with notable presence in those with high proliferative activity. This protein is primarily found in the cytoplasm and is associated with the mitotic spindle highlighting its specific involvement in cell division processes.
Biological function summary
MIOS serves as a component of the GATOR2 complex which plays an important role in the regulation of the mTORC1 pathway a central pathway in cell growth and metabolism. As part of this complex MIOS regulates amino acid sensing and signal transduction necessary for cellular responses to nutrient availability. This ensures that cells grow and divide only in environments that support such activities.
Pathways
MIOS interacts significantly within the mTOR signaling pathway. The GATOR2 complex where MIOS plays a part facilitates the regulation of mTORC1 by interacting with other proteins such as DEPDC5 and SESTRIN2. This pathway influences a variety of cellular processes including protein synthesis and autophagy. MIOS through its function in the complex affects the integration of growth signals and ultimately impacts cellular metabolism and homeostasis.
Disruptions in MIOS function connect to cancer and metabolic diseases given the involvement in the mTORC1 pathway. Aberrant MIOS expression or regulation may contribute to uncontrolled cell growth observed in some cancers where the mTOR pathway often shows hyperactivation. Additionally MIOS's role in nutrient sensing and metabolism links it to obesity and diabetes where the mTOR pathway's balance is disrupted. These conditions highlight MIOS's broader implications in disease when its pathways or interacting proteins such as GATOR2 components become dysregulated.