Abcam, ab265883, Human TBKBP1 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TBKBP1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 6 and 4 bp insertion in exon 6. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 6 and 4 bp insertion in exon 6,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TBKBP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TBKBP1 also known as TANK-Binding Kinase 1 Binding Protein 1 is a protein involved in several cellular processes. It has a mass of around 61 kDa. TBKBP1 is widely expressed in various tissues including the heart brain liver and kidney. Mechanically TBKBP1 interacts with TBK1 (TANK-binding kinase 1) influencing signal transduction and the regulation of innate immune responses.
Biological function summary
TBKBP1 functions as a modulator of immune signaling pathways. It often associates with complexes involving adaptor proteins such as TANK and IKK epsilon (IKKε). TBKBP1 plays a significant role in regulating antiviral responses by facilitating the activation of transcription factors that induce the expression of interferon-stimulated genes. These activities highlight its involvement in the regulation of the immune system's response to viral infections.
Pathways
TBKBP1 participates in the type I interferon signaling pathway and NF-kB signaling pathway. Within these pathways it works closely with proteins like TBK1 and IKKε contributing to cellular responses against viral and inflammatory stimuli. Through these interactions TBKBP1 helps modulate the production of cytokines and other mediators of inflammation.
TBKBP1 has connections to neurodegenerative diseases and cancer. Alterations in its normal function can result in impaired immune responses potentially contributing to the development of conditions like amyotrophic lateral sclerosis (ALS) and various forms of cancer. The interaction between TBKBP1 and TBK1 links it to pathological processes involving aberrant immune signaling and cellular stress responses.