Abcam, ab265959, Human PPP1R15B knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PPP1R15B KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PPP1R15B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PPP1R15B also known as GADD34 is a regulatory subunit of protein phosphatase 1 (PP1). It functions mechanically by associating with PP1 to regulate the dephosphorylation of the eukaryotic initiation factor 2 alpha (eIF2α). This interaction is essential for controlling protein synthesis especially during stress conditions. The molecular mass of PPP1R15B is approximately 74 kDa. Expression of PPP1R15B occurs in various tissues with high levels in brain liver and kidney tissues.
Biological function summary
The regulatory action of PPP1R15B on eIF2α impacts protein synthesis by reversing the phosphorylation which occurs in response to cellular stress. It functions as part of a multi-protein complex that includes PP1 and possibly other proteins involved in cellular stress responses. As a result it helps in the recovery of protein translation during periods of endoplasmic reticulum stress contributing to cell survival pathways under transient stress conditions.
Pathways
PPP1R15B plays a significant role in the integrated stress response pathway by modulating protein synthesis. It participates in the unfolded protein response (UPR) which is important when cells encounter endoplasmic reticulum stress. Through its interaction with eIF2α and other associated components like PP1 PPP1R15B facilitates cellular adaptation to changing environmental stressors.
PPP1R15B has been implicated in diseases such as neurodegenerative disorders and certain types of cancer. Deregulation of its function can lead to aberrant protein synthesis control which results in pathological outcomes. In neurodegenerative diseases its interaction with proteins like eIF2α is significant affecting neuronal cell survival under stress. In cancer altered expression or function of PPP1R15B may contribute to tumor progression due to its role in managing cellular stress responses and apoptosis.