Abcam, ab265994, Human WDR59 knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
WDR59 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 47 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 1 and 47 bp deletion in exon 1 and Insertion of the selection cassette in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-WDR59, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WDR59 also known as WD repeat domain 59 functions as a member of the WD repeat protein family. This protein weighs approximately 61 kDa. It plays a role as a part of the GATOR2 complex and regulates mTORC1 signaling. Researchers have observed its expression in various tissues including the brain and liver reflecting its broad functional importance in cellular activities.
Biological function summary
WDR59 interacts with various proteins and facilitates amino acid sensing by mTORC1. It forms part of a subcomplex known as GATOR2 which acts in opposition to GATOR1 to activate mTORC1 when cellular amino acid levels are sufficient. WDR59's involvement in amino acid signaling highlights its role in nutrient sensing pathways influencing cellular growth and metabolism.
Pathways
WDR59 affects mTOR signaling and the PI3K-Akt pathway. By participating in mTORC1 regulation it helps dictate cell growth proliferation and survival in response to nutrient availability. Its interaction with other GATOR2 components such as WDR24 and SEH1L illustrates its integration in nutrient sensing and metabolic regulation pathways.
Changes in WDR59 function associate with certain cancers especially where mTOR signaling malfunctions are part of the pathology. For instance errant mTOR pathway signaling may contribute to tumor growth. Additionally WDR59 might link to neurodevelopmental disorders considering its brain expression and roles in cellular signaling pathways. Interactions with proteins like DEPDC5 can highlight these associations as both are involved in mTOR pathway regulation.