Abcam, ab266021, Human NCAPD3 (hCAP-D3) knockout HeLa cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
NCAPD3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1,
Disease:Adenocarcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NCAPD3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HCAP-D3 also known as chromosome-associated protein D3 or CAP-D3 plays a role in the regulation of chromatid structure during cell division. With a molecular mass of approximately 160 kDa this protein is a component of the condensin II complex. hCAP-D3 is expressed in various tissues throughout the body prominently in cells that are dividing. Its expression changes can influence chromosomal segregation which is essential for proper cell cycle progression.
Biological function summary
HCAP-D3 influences the stabilization of chromosome architecture. It is a part of the condensin II complex working with other proteins like hCAP-H2 and hCAP-G2 to facilitate mitotic chromatid condensation. The correct function of hCAP-D3 during mitosis ensures proper separation of sister chromatids maintaining genomic integrity. The protein's mechanic role is important in preventing aneuploidy which can lead to various cellular malfunctions.
Pathways
HCAP-D3 participates in the mitotic cell cycle pathway significantly affecting chromosomal condensation processes. Its interaction with proteins like SMC2 and SMC4 is critical for the optimal function of the condensin II complex. Additionally hCAP-D3 engages in pathways associated with DNA repair and replication highlighting its importance in cell division and genomic stability. These interactions demonstrate its integration within cellular processes that preserve cell health.
HCAP-D3 associates with certain cancers due to its role in genomic stability. Aberrations in hCAP-D3 expression or function can lead to improper chromosomal segregation contributing to oncogenesis. Specifically research links hCAP-D3 to colorectal cancer where its altered activity may drive tumor progression. The protein also has connections to proteins involved in the control of mitotic checkpoints such as Aurora B highlighting its relevance in maintaining cell cycle fidelity and preventing disease.