Abcam, ab266064, Human CPNE1 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
CPNE1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 26 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 26 bp deletion in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-CPNE1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target CPNE1 also known as Copine-1 is a calcium-dependent phospholipid-binding protein. It weighs approximately 59 kDa and is expressed in various tissues including the brain heart and skeletal muscle. CPNE1 has eight conserved regions which include two C2 domains and a von Willebrand factor A domain enabling its function in the cell.
Biological function summary
CPNE1 interacts with membrane lipids and proteins playing a significant role in diverse cellular processes. It is part of a larger complex associated with membrane trafficking and cytoskeletal organization. CPNE1 can influence cellular processes like cell division and motility through its interactions with other proteins often regulating intracellular signaling pathways.
Pathways
CPNE1 is involved in calcium-mediated signaling and the phosphoinositide 3-kinase (PI3K) pathway. The protein interacts with Akt a downstream target of the PI3K pathway therefore influencing cell survival and growth. These interactions underline CPNE1's role in essential cellular functions linking it with other proteins like phospholipase C.
CPNE1 has associations with cancer progression and neurodegenerative diseases. It interacts with proteins like p53 which play important roles in cell cycle regulation and apoptosis. In cancer altered CPNE1 activity can disrupt normal cell signaling potentially leading to uncontrolled cell growth. In neurodegenerative diseases its involvement in cell signaling pathways may contribute to neuronal dysfunction or death.