Abcam, ab266080, Human TMED9 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TMED9 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TMED9, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TMED9 also known as Transmembrane Emp24 Domain-Containing Protein 9 carries out important mechanical roles in cellular processes. TMED9 has a mass of about 25 kDa. It functions as a part of the p24 family of proteins known to help in the transportation of proteins between the endoplasmic reticulum and the Golgi apparatus. TMED9 contains domains that are typical for vesicular transport proteins. This protein expresses largely in various tissues including the liver and kidney where protein transport demands are high.
Biological function summary
The TMED9 protein engages in the regulation of protein transport and maturation within cells. It associates with other p24 proteins to form a complex that modulates trafficking of cargo molecules to their correct destinations. This complex plays a role in ensuring accurate membrane formation and lipid metabolism affecting cellular homeostasis. The interaction of TMED9 with other proteins especially within the p24 family is essential for its functional roles in the secretory pathway.
Pathways
TMED9 engages in the endoplasmic reticulum to Golgi transport pathway. This pathway is responsible for the precise delivery of proteins to the Golgi where they undergo further modification. Its interactions with other p24 family members like TMED2 support its role in vesicular transport. Additionally TMED9 might play roles in lipid metabolism intersecting with pathways where proteins such as apolipoproteins participate influencing cellular lipid balance and energy storage.
TMED9 involvement has been observed in conditions like liver diseases and certain types of cancer. TMED9 dysregulation impacts protein transport pathways contributing to cellular dysfunctions seen in liver cirrhosis. Also in certain cancers TMED9 interacts with proteins like TMED10 potentially affecting cell proliferation and migration. Understanding its role could help in developing therapeutic strategies targeting these pathways in disease contexts.