Abcam, ab266083, Human MTCH2 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MTCH2 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MTCH2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MTCH2 also known as Mitochondrial Carrier Homolog 2 serves as a protein involved in mitochondrial function. It has a mass of approximately 33 kDa. This protein sees high levels of expression in adipose tissue liver heart and skeletal muscle indicating its role in energy metabolism and homeostasis. MTCH2 localizes to the outer mitochondrial membrane where it impacts the transfer of metabolic substrates and signals across the mitochondrial barrier.
Biological function summary
MTCH2 plays a significant role in regulating mitochondrial apoptosis and metabolism. It is involved in the control of lipid and glucose metabolism essential for maintaining cellular energy balance. MTCH2 forms part of a larger protein complex associated with the mitochondrial outer membrane and interacts with several key regulators of apoptosis including tBID. Its function is important to maintaining cell survival and regulating programmed cell death in response to metabolic stress.
Pathways
The regulatory role of MTCH2 integrates it into the mitochondrial apoptosis and lipid metabolism pathways. MTCH2 acts synergistically within the apoptosis pathway by influencing the activity of proteins such as Bax. In the lipid metabolism pathway MTCH2 coordinates with proteins like CD36 to regulate fatty acid uptake and oxidation connecting cellular energy demands with supply.
MTCH2 has relevance to metabolic conditions and neurodegenerative diseases. In obesity alterations in MTCH2 function affect energy metabolism and fat accumulation. In Alzheimer's disease MTCH2 interaction with amyloid precursor protein regulates mitochondrial function and could contribute to neuronal death. MTCH2 associating with tBID and Bax further implicates its role in the progression of these conditions.