Abcam, ab266106, Human KLHDC3 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
KLHDC3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-KLHDC3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
KLHDC3 also known as Kelch Domain Containing 3 is a protein with a mass of approximately 30 kDa. It is characterized by the presence of a Kelch domain which enables protein-protein interactions. It is expressed in various tissues with notable presence in the brain and testes. This protein is part of the Kelch domain-containing family which often plays roles in scaffolding and organizing protein complexes within cells.
Biological function summary
KLHDC3 participates in cellular processes such as transcriptional regulation and cell cycle control. It is involved in forming protein complexes that modify transcriptional responses. These complexes interact with a variety of transcription factors impacting gene expression. KLHDC3 also regulates the stability and degradation of certain proteins by acting as an adapter for ubiquitination processes.
Pathways
KLHDC3 plays a role in critical pathways like the ubiquitin-proteasome system and the Wnt signaling pathway. It interacts with proteins involved in these pathways such as Beta-catenin influencing cellular growth and development. The ubiquitin-proteasome system is essential for protein turnover while Wnt signaling regulates gene transcription that affects embryogenesis and cell proliferation.
KLHDC3 has been linked to disorders like neurodegenerative diseases and certain cancers. Aberrant functioning of KLHDC3 or its related pathways can disrupt normal cellular processes leading to disease development. For example its interaction with Beta-catenin can influence pathways associated with tumor progression. Further research aims to understand how KLHDC3 modulations contribute to disease pathology and may guide therapeutic approaches.