Abcam, ab266162, Human PPM1G knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PPM1G KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PPM1G, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PPM1G also known as protein phosphatase 1G is a member of the protein phosphatase 2C (PP2C) family. This enzyme has a molecular mass of approximately 58 kDa. It localizes mainly in the nucleus and is expressed in various tissues including the brain testis and skeletal muscle. PPM1G functions as a serine/threonine phosphatase removing phosphate groups from target proteins and playing a role in the regulation of cellular stress responses and cell cycle progression.
Biological function summary
PPM1G contributes to several cellular functions by interacting with different substrates. As a component of the cellular machinery it is involved in processes such as RNA processing and regulation of mitosis. The enzyme participates in dephosphorylation events critical for the assembly and disassembly of the spliceosome complex which is important for accurate mRNA splicing. PPM1G's activity helps maintain the proper function of these cellular processes ensuring the stability and output of gene expression.
Pathways
Several significant cellular activities are influenced by PPM1G's regulatory mechanisms. It is implicated in the mitogen-activated protein kinase (MAPK) signaling pathway and the cell cycle regulation pathway. These pathways are vital for controlling cell growth and division. In these contexts PPM1G interacts with various other proteins such as p53 and cyclin-dependent kinases (CDKs) linking it to cell cycle checkpoints and stress responses. These interactions allow PPM1G to regulate and coordinate cellular responses to external and internal signals.
PPM1G has connections with illnesses like cancer and neurodegenerative diseases. In cancer its role in cell cycle regulation can affect tumor growth and progression often depending on its interactions with proteins like CDK. In neurodegenerative disorders PPM1G might play a part due to its involvement in RNA processing and protein dephosphorylation processes which are critical for neuron function. Any dysregulation in these interactions may contribute to disease pathology making PPM1G a point of interest for understanding and potentially treating these conditions.