Abcam, ab266234, Human SLC7A1 (CAT1) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SLC7A1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 35 bp deletion in exon 3. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 35 bp deletion in exon 3

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC7A1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CAT1 also known as cationic amino acid transporter 1 or SLC7A1 is an integral membrane protein that facilitates the uptake of cationic amino acids such as arginine lysine and ornithine. CAT1 is a member of the solute carrier family 7 and has a molecular mass of approximately 68 kDa. It expresses widely in various tissues with particularly high levels in the liver lung and skeletal muscle. As a transporter CAT1 plays an essential role in amino acid homeostasis by mediating amino acid transport across the cell membrane.
Biological function summary
CAT1 influences nitric oxide production by regulating arginine availability a substrate for nitric oxide synthase. By controlling amino acid imports it affects cellular signaling and metabolism. CAT1 does not function as part of a larger complex but interacts with other transporters to maintain cellular amino acid balance. CAT1's role extends to impacting cell proliferation as cationic amino acids are important for growing tissues.
Pathways
CAT1 has been implicated in the nitric oxide synthesis pathway where it contributes by sustaining intracellular arginine levels. This involvement is critical to vascular function and immune response regulation. CAT1 also participates in mTOR signaling by providing necessary amino acids like arginine which influence protein synthesis and cellular growth. In these pathways CAT1 connects with proteins such as mTOR and nitric oxide synthase assisting in integral cellular functions.
CAT1 links to cardiovascular conditions through its role in nitric oxide production which affects blood pressure regulation and endothelial function. Dysfunction or altered expression of CAT1 can lead to cardiovascular abnormalities due to impaired nitric oxide synthesis. CAT1 is also implicated in cancer as its impact on arginine availability affects cell proliferation and tumor growth. In this context CAT1 interacts with oncogenes and tumor suppressor proteins that modulate growth-related pathways.