Abcam, ab266243, Human MZT2B knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MZT2B KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 29 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 29 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MZT2B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MZT2B protein also known as Mitotic Spindle-Organizing Protein 2B is a component of the mitotic spindle apparatus. It plays a role in the organization of microtubules during cell division. MZT2B has a molecular mass of approximately 20 kDa. It is expressed in various tissues with prominent expression in proliferative tissues such as those in the reproductive system and certain areas of the brain.
Biological function summary
The MZT2B protein contributes to the proper formation and function of the mitotic spindle. It is an integral part of the gamma-tubulin ring complex which is critical for microtubule nucleation at centrosomes. This complex ensures the stability and anchoring of microtubules facilitating accurate and equal chromosome segregation during mitosis. Proper spindle assembly is essential for optimal cell division and proliferation.
Pathways
The MZT2B protein plays a prominent role in the mitotic spindle assembly pathway and the cell cycle checkpoint pathway. It associates with proteins like gamma-tubulin and other components of the spindle assembly checkpoint machinery ensuring that all chromosomes are precisely aligned and attached to spindle fibers before proceeding to the next phase of cell division. The interplay between MZT2B and these pathways ensures successful mitosis and genomic stability.
Deregulation of MZT2B has been linked to various types of cancer including breast and ovarian cancer. Abnormalities in the function or expression of MZT2B can lead to chromosomal instability which is a hallmark of cancerous transformations. Changes in expression levels of MZT2B may also affect interactions with proteins like p53 an important tumor suppressor influencing cancer progression and treatment responses.