Abcam, ab266303, Human NASP knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
NASP KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 27 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 27 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NASP, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NASP also known as Nuclear Autoantigenic Sperm Protein functions as a histone chaperone. With a molecular mass of approximately 75 kDa NASP assists in the proper assembly and disassembly of nucleosome structures by binding to histones H1 and H2A-H2B. This protein mainly expresses in rapidly dividing tissues especially in the testes but also presents in the thymus and bone marrow. NASP maintains the supply of free histones aiding in DNA replication and transcription processes.
Biological function summary
NASP plays an essential role in chromatin organization and dynamics during the cell cycle. As part of the histone transport complex it stabilizes histones and prevents their aggregation. NASP facilitates proper chromatin assembly necessary for DNA packaging and regulation of gene expression. This activity ensures the accurate transmission of genetic information during cell division impacting processes essential for cell proliferation and function.
Pathways
NASP operates within the chromatin assembly and nucleosome remodeling pathways. It coordinates with other chaperones like NAP1 in chromatin assembly factor-1 (CAF-1) complex. These pathways are integral to DNA replication and repair directly linking NASP to cellular processes critical for maintaining genome integrity. The interaction with importin beta further indicates NASP's role in nuclear import pathways emphasizing its involvement in both nuclear organization and cellular regulation.
NASP associates with cancer and infertility. The aberrant expression of NASP is observed in various tumors implying its contribution to tumorigenesis and proliferation. Its association with other histone chaperones such as ASF1 highlights its potential role in oncogenic pathways. Furthermore alterations in NASP expression affect sperm development linking it to male infertility and emphasizing its necessity in reproductive biology.