Abcam, ab266305, Human TIMM17A (TIM17) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TIMM17A KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 2 and Insertion of the selection cassette in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TIMM17A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TIMM17A also known as TIM17A or simply TIM17 is a protein component of the mitochondrial inner membrane translocase complex. It is involved in the import of nuclear-encoded proteins into the mitochondria. With a molecular mass of approximately 19 kDa TIMM17A is primarily expressed in the inner mitochondrial membrane. This protein forms part of the TIM23 complex which mediates the translocation of presequence-containing preproteins into the mitochondrial inner membrane or matrix.
Biological function summary
The TIMM17A protein facilitates the import of essential mitochondrial proteins securing proper mitochondrial function and energy production. TIMM17A as part of the TIM23 translocase complex cooperates closely with other TIM proteins such as TIMM50 and TIMM23 to ensure protein import is efficient and targeted. Its role in maintaining mitochondrial integrity also supports cellular metabolism and the regulation of apoptotic processes which are processes responsible for programmed cell death.
Pathways
The TIMM17A protein plays a significant role in the mitochondrial protein import pathway. It interacts with other key proteins like HSP70 and mtHSP70 which manipulate peptide folding and assist in pulling preproteins through the TIM23 channel. The proper import of these proteins is essential for the oxidative phosphorylation pathway which is important for ATP production. This pathway enables ATP synthesis by transferring electrons through a sequence of electron transport chain complexes located in the mitochondrial inner membrane.
Research associates TIMM17A dysfunction with mitochondrial myopathy a disorder characterized by muscle weakness and dysfunction due to impaired mitochondrial performance. Studies also link alterations in TIMM17A to certain neurodegenerative disorders such as Parkinson's disease where mitochondrial dysfunction plays an important role. The protein's interaction with other import machinery members during these conditions like TIMM44 highlights its importance in maintaining mitochondrial health and function.