Abcam, ab266448, Human CYSTM1 (ORF1 FL49) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
CYSTM1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-CYSTM1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein ORF1 FL49 sometimes referred to in short as FL49 is an important component in biological processes at the cellular level. Structurally it has a molecular mass that has been experimentally determined to be approximately 55 kDa. Scientists have observed its expression in several tissues with notable presences in the liver and brain. This protein is a part of the greater ORF1 motif and is important to the individual cellular mechanisms where it is located.
Biological function summary
ORF1 FL49 has been shown to modulate various intracellular processes. It often functions in collaboration with a complex of other proteins to influence mRNA translation and stability. Researchers observed its involvement in regulating RNA processing where it affects both the synthesis and degradation of RNA. Studies have linked this protein to the energy metabolism pathways within the cell emphasizing its critical role in maintaining cellular homeostasis.
Pathways
Understanding ORF1 FL49 provides valuable insight into how cellular signaling and energy regulation occurs. This protein plays a significant part in the mTOR signaling pathway which affects cell growth and nutrient sensing. Furthermore it interacts with proteins such as S6K1 and AMPK integrating signals that influence metabolic activity and stress responses. This integration highlights the protein's contribution to maintaining energy balance within the organism.
Mutations or altered regulation of ORF1 FL49 have been associated with metabolic diseases and neurodegenerative conditions. Disruptions in the normal function of this protein can lead to abnormal cell growth and impaired energy management significantly contributing to disorders such as metabolic syndrome and Alzheimer's disease. In metabolic syndrome researchers have found that the protein's pathway interactions particularly with AMPK play a pivotal role in disease progression. In Alzheimer's for instance its relationship with mTOR signaling suggests that ORF1 FL49 may influence disease onset and progression particularly through its impact on neuronal cell health.