Abcam, ab266552, Human ZNHIT1 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
ZNHIT1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 215 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 215 bp insertion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ZNHIT1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZNHIT1 also known as Zinc finger HIT-type containing 1 is a protein that plays a role in the assembly of multiprotein complexes. It has a molecular mass of approximately 29 kDa. This protein is found expressed in various tissues including the brain heart and liver suggesting its involvement in a range of biological functions. ZNHIT1 contains zinc finger motifs which are key for its interaction with other proteins facilitating its mechanical role in cellular processes.
Biological function summary
The interaction of ZNHIT1 with other protein units forms part of the histone and transcription machinery. It is a component of the U3 small nucleolar ribonucleoprotein (snoRNP) complex which is essential for the biogenesis of ribosomes. Through its zinc finger domains ZNHIT1 interacts with proteins such as NOLC1 aiding in the functional assembly of ribosome-related structures. The activity of ZNHIT1 within this context points to its importance in maintaining protein synthesis and gene regulation.
Pathways
ZNHIT1 is involved in the metabolic and cellular processes of ribosome assembly and gene expression. In the context of these pathways its interaction with NOLC1 facilitates alterations in nucleolar activity which can impact protein synthesis. ZNHIT1 can influence pathways such as the assembly of the pre-rRNA complex and is also linked to transcriptional regulation alongside proteins like SMARCC1 within epigenetic modification routes.
ZNHIT1 has connections with certain cancers and neurodegenerative conditions. Its regulatory roles in gene expression and ribosome production can contribute to pathologies like hepatocellular carcinoma where aberrations in protein synthesis are common. Altered function or expression of ZNHIT1 can also associate with neurological disorders interconnected through proteins such as AATF which is implicated in neuronal survival. Understanding ZNHIT1's involvement in these pathways can offer insights into potential therapeutic strategies.