Abcam, ab266582, Human ATP5J2 knockout HEK-293T cell line

Size: 1000000Cells / vial
ATP5MF KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp insertion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ATP5MF, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
'ATP5J2' also known as ATP synthase-coupling factor 6 mitochondrial is a protein involved in the synthesis of ATP the primary energy carrier in cells. This protein integrates into the ATP synthase complex specifically the F0 part where it helps regulate proton flow. ATP5J2 is approximately 11 kDa in mass and appears predominantly in mitochondria the powerhouse of cells. It expresses widely in numerous tissues highlighting an important role in cellular energy metabolism fundamental to many physiological processes.
Biological function summary
ATP5J2 functions as an accessory subunit within the ATP synthase complex which is responsible for ATP production during oxidative phosphorylation. By participating in the regulation of proton translocation through the mitochondrial membrane ATP5J2 helps maintain the proton motive force necessary for ATP generation. The protein's involvement in metabolic energy conversion underlines its role in effective cellular respiration.
Pathways
ATP5J2 aligns with the oxidative phosphorylation pathway an essential metabolic route for energy production. It associates with other subunits of the ATP synthase complex such as ATP5B and ATP5O to fulfill regulatory roles in ATP synthesis. Additionally ATP5J2 has interactions within the mitochondrial electron transport chain contributing to the efficient function of this pathway and sustaining the energy requirements of cells.
Defects in ATP5J2 impact mitochondrial function linking it to mitochondrial diseases which often present with muscle weakness and neural disorders. Moreover abnormalities in ATP5J2 may associate with metabolic syndromes affecting global energy metabolism. These conditions highlight potential interactions with proteins like NDUFS1 another component of the electron transport chain where disturbances can exacerbate the impact of energy deficiencies on cellular health.