Abcam, ab266612, Human RPL22 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RPL22 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RPL22, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RPL22 also known as Ribosomal Protein L22 is a component of the 60S subunit of ribosomes playing an important role in protein synthesis. It has an approximate molecular weight of 16 kDa. Expression of RPL22 occurs in various tissues but it shows high levels in the brain liver and spleen. The protein's structural role within the ribosome allows it to contribute to the assembly and stability necessary for ribosomal function.
Biological function summary
RPL22 contributes to ribosomal biogenesis by being part of a larger ribonucleoprotein complex. It participates in the assembly of the 60S subunit of ribosomes which is essential for translation. The proper function of RPL22 ensures accurate and efficient polypeptide elongation during protein synthesis. Being a part of the ribosomal machinery RPL22 impacts cell proliferation and growth as ribosomes are critical for producing proteins needed for cell development and function.
Pathways
RPL22 plays a role in the translation machinery impacting the protein synthesis pathway. It interacts with other ribosomal proteins such as RPL5 and RPL10 which together facilitate the translation of mRNA into protein. Additionally RPL22 has relevance in the mTOR signaling pathway an important regulator of cell growth and proliferation where it affects processes by influencing ribosomal output and protein translation directly.
RPL22 links to conditions such as cancer and Diamond-Blackfan anemia. Its aberrant expression or mutations can affect ribosome function contributing to tumorigenesis due to uncontrolled protein synthesis and cell proliferation. In particular studies show that leukemia and solid tumors may involve altered RPL22 activity. In Diamond-Blackfan anemia disrupted ribosomal biogenesis due to mutations in RPL22 associates with bone marrow failure. The protein's interactions with other ribosomal proteins like RPS19 help illustrate its potential involvement in these diseases.