Abcam, ab266694, Human MPDU1 (SL15) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MPDU1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and Insertion of the selection cassette in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MPDU1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SL15 also known as Signal Ligand 15 is an important protein involved in cellular signaling. It exhibits a molecular mass around 58 kDa and shows widespread expression in immune cells particularly in macrophages and dendritic cells. The protein consists of several domains that aid in its role in transmission of signals across the cell membrane triggering intracellular responses. Its expression is highest in tissues involved in immune responses such as the spleen and lymph nodes where it plays a role in the modulation of immune functions.
Biological function summary
SL15 functions as a pivotal element in initiating immune cell activation. It is a component of the SL15-Complex which interacts with other receptors on the cell surface to mediate downstream signaling cascades. This interaction can lead to changes in gene expression that promote inflammatory responses. Working in tandem with co-receptors SL15 plays a central role in regulating the intensity and duration of immune reactions which are critical for maintaining homeostasis and defense against pathogens.
Pathways
SL15 is essential in the regulation of the NF-kB signaling pathway which is vital for immune and stress responses. Its involvement in this pathway connects it to a network of other proteins such as the TRAF family that facilitate the NF-kB transcriptional activation. SL15 also participates in the MAPK signaling pathway where it influences cell proliferation and differentiation. These pathways ensure that the cell responds appropriately to external stimuli and maintain cellular balance.
SL15 has garnered interest due to its association with autoimmune diseases like rheumatoid arthritis and inflammatory bowel disease. Abnormal SL15 signaling may result in improper immune responses leading to chronic inflammation. It shows interactions with proteins like IL-6 and TNF-alpha which are mediators of inflammation and have been implicated in disease progression. Targeting SL15 in therapeutic interventions might offer new avenues for managing these conditions.