Abcam, ab266709, Human WIBG (PYM) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PYM1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 1 and 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 1 and 1 bp insertion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PYM1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PYM also known as PYM1 or Partner of Y14 and Magoh is a mechanistic component involved in the regulation of nonsense-mediated mRNA decay (NMD). It has a molecular mass of approximately 29 kDa. PYM is mainly expressed in the cytoplasm of cells particularly in regions with high cellular turnover such as the brain and testis. PYM interacts with the exon junction complex adjusting mRNA metabolism by binding to key components Y14 and Magoh facilitating mRNA surveillance.
Biological function summary
The PYM protein has significant influence on the mRNA surveillance machinery working as a modulator of NMD pathways. PYM is closely linked with the exon junction complex functioning with Y14 and Magoh to ensure mRNA molecules are properly processed for translation. It does not work alone; it forms part of a specialized complex that recognizes faulty mRNA with premature stop codons ensuring the upkeep of cellular mRNA quality.
Pathways
The PYM protein functions within the mRNA surveillance pathway which preserves integrity of gene expression. PYM interacts with Upf1 which plays a major role in detecting and initiating the degradation of faulty mRNA. It also connects with components of the mRNA export pathway ensuring only properly spliced and processed transcripts leave the nucleus. The partnership between PYM and other exon junction complex proteins contributes significantly to maintaining efficient and accurate gene expression protocols.
Errors in PYM function associate with neurological disorders due to its expression in the brain. Mutations or dysregulation here can affect mRNA quality control leading to ailments such as intellectual disability. PYM's interactions with Upf1 link it to disorders where NMD pathway errors occur further highlighting its importance in maintaining nervous system health. These associations highlight the potential for PYM as a target for therapeutic interventions in related conditions.