Abcam, ab266737, Human TTLL12 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TTLL12 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TTLL12, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TTLL12 also known as Tubulin Tyrosine Ligase Like 12 is an enzyme that belongs to the tubulin tyrosine ligase-like (TTLL) family. This protein functions mechanically by catalyzing the polyglutamylation of target proteins especially tubulin impacting microtubule stability and interaction. TTLL12 has a mass of approximately 60 kDa and is predominantly expressed in the brain and testis although its expression is noted in other tissues as well. The involvement of TTLL12 in these tissues suggests its significance in specialized cellular processes.
Biological function summary
TTLL12 influences neuronal and cellular functions through its capacity to alter the microtubule network. It participates in the polyglutamylation activity that regulates microtubule dynamics synaptic stability and intracellular transport playing critical roles in neurodevelopment and cellular organization. Although TTLL12 itself does not commonly form large complexes its enzymatic activity impacts other microtubule-modifying complexes indirectly affecting cellular architecture and signaling pathways.
Pathways
TTLL12 participates notably in the microtubule-network pathway and the regulation of cytoskeleton organization. Its activity modulates the polymerization and depolymerization dynamics of microtubules impacting cell cycle and neuronal signaling. TTLL12's function is related to other tubulin-modifying enzymes such as TTLL1 and TTLL5 which share a role in tubulin modification thereby affecting cellular processes like mitosis and intracellular trafficking.
TTLL12's aberrant activity or expression has connections to neurodegenerative diseases and male infertility. For example its dysfunction in the brain is associated with tauopathies where improper microtubule regulation contributes to disease pathology. Additionally TTLL12's function relates to proteins like tau and other microtubule-associated proteins (MAPs) which play roles in maintaining neuronal structure and function. Understanding its connections to these conditions may shed light on potential therapeutic strategies.