Abcam, ab266751, Human NPM3 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
NPM3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NPM3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The nucleophosmin/nucleoplasmin family member 3 (NPM3) also known as nucleoplasmin 3 is a small acidic protein with a molecular mass of approximately 22 kDa. NPM3 localizes mainly in the nucleolus where it participates in various nuclear processes. The protein is expressed widely with higher levels in tissues such as the thymus and testes. It belongs to the histone chaperone family and shares a structural similarity with nucleophosmin (NPM1) promoting its involvement in chromatin assembly and disassembly.
Biological function summary
NPM3 plays a role in the regulation of chromatin structure and gene expression. It forms complexes with other proteins to facilitate the proper folding and deposition of histone proteins onto DNA. This process is essential for maintaining the dynamic nature of chromatin allowing genes to be accessed and transcribed as needed. NPM3's function contributes to the efficiency of DNA replication and repair mechanisms within the cell potentially affecting cell cycle progression.
Pathways
NPM3 is involved in key cellular mechanisms such as DNA replication and repair as well as the regulation of transcription. It interacts with proteins involved in the nucleosome assembly pathway including NPM1 which enhances its role in these critical tasks. NPM3's association with these pathways highlights its influence on maintaining genomic integrity and proper cellular function by ensuring accurate DNA duplication and protection from genomic instability.
NPM3's dysfunction or altered expression can contribute to the development of cancer particularly hematological malignancies like leukemia. Studies have shown a connection between NPM3 and the oncogenic processes involving NPM1 which is frequently mutated in certain cancer types. Additionally alterations in NPM3 expression have been observed in neural disorders such as Alzheimer's disease implicating its role in neurodegenerative processes possibly through its impact on chromatin dynamics and neuronal gene expression regulation.