Abcam, ab266761, Human COQ9 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
COQ9 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-COQ9, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
COQ9 also known as coenzyme Q9 homolog is a protein integral to coenzyme Q (ubiquinone) biosynthesis. It has a molecular mass of approximately 30 kDa. COQ9 expresses mainly in mitochondria the energy centers of cells. This localization aligns with its role in the mitochondrial respiratory chain where it assists in ubiquinone production essential for electron transport and ATP generation.
Biological function summary
COQ9 operates within a multi-subunit complex called the CoQ biosynthesis complex. This complex supports the synthesis of coenzyme Q10 a molecule involved in cellular respiration. COQ9's function ensures the stability and efficiency of the CoQ biosynthesis process which is necessary for mitochondrial health and energy production. Its role is supported by interactions with other proteins in the complex fostering effective biosynthesis of this quinone compound.
Pathways
The COQ9 protein is an important player in the ubiquinone biosynthesis pathway and indirectly influences the electron transport chain. COQ9 associates closely with other COQ proteins such as COQ8 which collectively drive the coenzyme Q production essential for the electron transport chain's proper function. Defects in this pathway can disrupt cellular energy metabolism highlighting COQ9's significance.
Dysfunctions in COQ9 relate to conditions like primary coenzyme Q10 deficiency and mitochondrial encephalomyopathies. These disorders stem from inadequate coenzyme Q10 production leading to impaired mitochondrial function. COQ9 deficiencies can involve COQ2 mutations as both proteins participate in coenzyme Q10 biosynthesis further linking COQ9 to these pathological states.