Abcam, ab266775, Human SPAG7 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SPAG7 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SPAG7, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPAG7 also known as Sperm Associated Antigen 7 is a protein with an approximate mass of 26 kilodaltons. It is mainly expressed in the testis with additional expression noted in other tissues at lower levels. Mechanically SPAG7 functions in the regulation of cellular processes associated with spermatogenesis playing a role in the assembly and reorganization of microtubule structures.
Biological function summary
SPAG7 enhances the development and function of sperm cells contributing significantly to male fertility. The protein participates in microtubule-related activities and likely interacts with other proteins to perform its roles effectively. Research suggests that SPAG7 does not form a complex on its own but it closely associates with other cellular components in sperm maturation.
Pathways
SPAG7 interacts with regulatory networks involved in cilia and flagella movement contributing to the sperm's motility. SPAG7 shows a relationship with proteins like dynein and kinesin which are important in microtubule dynamics and cellular transport processes. It plays a part in pathways such as the spermatogenesis pathway where it ensures proper function and motility of sperm cells through its interactions with other proteins.
SPAG7 has associations with male infertility which is often linked to defective spermatogenesis and sperm motility issues. Abnormal SPAG7 expression may disrupt cellular transport processes causing infertility. Although not directly implicated in diseases like cancer alterations in SPAG7 levels could potentially influence cancer-associated proteins through indirect pathways reflecting complex biological interactions.