Abcam, ab266799, Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
PIK3R2 KO cell line available to order. KO validated by Western blot. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PIK3R2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PI 3-kinase p85 beta also known as PIK3R2 is a regulatory subunit of phosphoinositide 3-kinases (PI3K). This protein has a molecular weight of approximately 85 kilodaltons. It expresses widely in various tissues including brain liver and skeletal muscle. The p85 protein form interacts with the catalytic subunit p110 allowing for the proper localization and activation of PI3K in response to various extracellular signals.
Biological function summary
The regulatory subunit PI 3-kinase p85 beta is responsible for maintaining the balance of PI3K activity within the cellular environment. It forms a dynamic complex with the catalytic subunit influencing downstream signaling pathways. The presence of p85 beta ensures the stability and function of the PI3K complex essential for processes such as cell growth survival and metabolism.
Pathways
This protein plays a significant role in the PI3K/AKT/mTOR signaling pathway and also contributes to insulin signaling. It partners with p110 and mediates the transmission of signals from receptor tyrosine kinases (RTKs) like insulin receptors. The regulation by p85 beta affects protein synthesis and glucose uptake highlighting its function in cellular energy management.
Dysregulation of PI 3-kinase p85 beta associates frequently with types of cancer and type 2 diabetes mellitus. Aberrant signaling through the PI3K pathway involving interactions between p85 beta and p110 contributes to oncogenesis. Similarly inappropriate regulation of insulin signaling links p85 beta with insulin resistance an important factor in type 2 diabetes where it may interact with proteins such as IRS-1 (Insulin Receptor Substrate 1) to influence disease progression.