Abcam, ab266802, Human RNF10 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
RNF10 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 1 and Insertion of the selection cassette in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon 1 and Insertion of the selection cassette in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RNF10, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RNF10 also known as RING finger protein 10 is an E3 ubiquitin-protein ligase that plays a role in attaching ubiquitin molecules to target substrates marking them for proteasomal degradation. RNF10 has a molecular weight of approximately 75 kDa. It is expressed in various tissues including the brain heart and skeletal muscle. RNF10 contains a RING finger domain important for its ligase activity allowing it to interact with E2 ubiquitin-conjugating enzymes.
Biological function summary
RNF10 participates in regulating cellular processes like neurodevelopment and muscle differentiation. It interacts with other proteins to form complexes that influence cell signaling pathways and transcription regulation. This protein's activities affect neuronal growth and synaptic plasticity important for learning and memory. RNF10 also has implications in muscle cells where it modulates the expression of genes related to muscle function.
Pathways
RNF10 takes part in the MAPK signaling pathway which plays a role in cell proliferation and differentiation. In this pathway RNF10 interacts with proteins such as MEK and ERK allowing it to influence downstream signaling processes. Additionally RNF10 has connections to the Wnt signaling pathway impacting gene expression patterns important for development. These interactions highlight its role in orchestrating complex cellular responses.
RNF10 is linked to neurological and muscular conditions. Its involvement in neurodevelopment suggests a connection to neurodegenerative diseases like Alzheimer's where protein degradation processes may go awry. In muscular disorders RNF10 may interact with proteins like MYOD1 influencing muscle differentiation and regeneration. These associations highlight the importance of understanding RNF10's role in healthy and diseased states.