Abcam, ab266832, Human MRPL50 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MRPL50 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MRPL50, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRPL50 also known as Mitochondrial Ribosomal Protein L50 is a component of the mitochondrial ribosome. With a molecular mass of approximately 16.5 kDa MRPL50 is located within the mitochondria the energy-producing centers of the cell. It forms part of the large 39S subunit of mitochondrial ribosomes and is essential for protein synthesis within mitochondria. Expression of MRPL50 occurs in tissues with high energy demands like muscle and brain reflecting its role in mitochondrial function.
Biological function summary
MRPL50 plays an essential role in the formation and function of the mitochondrial ribosomal large subunit. A component of the mitochondrial ribosomal complex it contributes to translating mitochondrial mRNA into proteins necessary for oxidative phosphorylation. This function is critical for the maintenance of mitochondrial activity and by extension cellular energy metabolism. The interactions within the ribosomal complex help ensure the efficient synthesis of proteins encoded by mitochondrial DNA.
Pathways
The function of MRPL50 integrates into the oxidative phosphorylation and mitochondrial translation pathways. This protein is important for the synthesis of components in the electron transport chain a pivotal pathway in energy production. MRPL50 operates together with other mitochondrial ribosomal proteins in translating mitochondrial genes into functional proteins which link into pathways like those involving the NADH dehydrogenase complex.
Malfunctions or mutations in MRPL50 associate with mitochondrial diseases such as mitochondrial myopathy and neurodegenerative disorders. The role of MRPL50 in producing mitochondrial proteins links it to conditions where mitochondrial energy production is compromised. Related proteins in these diseases include those involved in the assembly and stability of the mitochondrial ribosomes as well as key constituents of the oxidative phosphorylation system.