Abcam, ab267157, Human MSX1 knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
MSX1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MSX1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MSX1 protein sometimes called Homeobox protein MSX-1 is a transcription factor with a mass of approximately 33 kDa. It plays a role in the regulation of embryonic development by controlling the expression of several genes. This protein is mainly expressed in tissues such as the limb buds craniofacial regions and developing teeth which are critical sites for its function during different stages of growth.
Biological function summary
MSX1 modulates a variety of developmental processes including craniofacial morphogenesis and limb formation. It operates as part of a DNA-binding complex that influences the transcription of target genes essential for proper tissue differentiation and formation. Through its activity MSX1 affects cell proliferation and apoptosis ensuring the accurate development of specific structures in the organism.
Pathways
MSX1 interacts with the WNT signaling and bone morphogenetic protein (BMP) pathways. In the WNT signaling pathway MSX1 modulates the expression of genes involved in the regulation of developmental processes and cell fate determination. It also interacts with BMP-related proteins playing an important role in skeletal formation and patterning demonstrating connections with other proteins like BMP4 and LEF1.
MSX1 has been implicated in the development of tooth agenesis and craniosynostosis. Research shows that mutations in the MSX1 gene can lead to these conditions affecting the normal formation and differentiation of skeletal elements. Furthermore MSX1 has connections with the protein PAX9 as together they contribute to the regulation of dental development and disruptions in their interactions often result in anomalies in tooth formation.