Abcam, ab267178, Human USP18 (UBP43) knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
USP18 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 97 bp deletion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 97 bp deletion in exon 4,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-USP18, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
UBP43 also known as USP18 is a deubiquitinating enzyme that is 43 kilodaltons in mass. This protein removes ubiquitin from ubiquitinated proteins which regulates their degradation and stability. UBP43 is expressed widely in the body with significant levels in the immune and nervous systems. Its alternate name USP18 reflects its inclusion in the ubiquitin-specific protease family highlighting its specific function in modulating protein ubiquitination.
Biological function summary
UBP43 regulates type I interferon signaling and affects immune response. As a negative feedback regulator it inhibits the interferon signaling pathway preventing unnecessary immune reactions. UBP43 does not form a part of a larger protein complex but acts independently to maintain cellular homeostasis. Its activity impacts the function of other proteins involved in response to viral infections.
Pathways
UBP43 plays an important role in the JAK-STAT signaling pathway interacting with proteins involved in cytokine signaling. It influences the pathway by inhibiting the action of interferon-stimulated genes therefore modulating the immune response. UBP43's interaction with ISG15 a ubiquitin-like modifier is also a critical aspect that affects other cellular processes like protein modification and response to inflammatory stimuli.
UBP43 is closely linked to autoimmune diseases and viral infections. Altered UBP43 activity can lead to improper immune responses contributing to conditions such as systemic lupus erythematosus. It also plays a role in the body's defense against viral infections where its dysregulation may affect the efficiency of antiviral responses. The interplay between UBP43 and ISG15 is of particular interest as changes in their interaction could provide insights into the development or progression of these disorders.