Abcam, ab267186, Human TRANK1 knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TRANK1 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 11 and 7 bp deletion in exon 11. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 11 and 7 bp deletion in exon 11,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TRANK1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TRANK1 also known as Tetratricopeptide Repeat And Ankyrin Repeat Containing 1 is a protein with a mass of approximately 190 kDa. This protein features tetratricopeptide repeats which assist in protein-protein interactions. TRANK1 is expressed widely but higher levels appear in brain tissues. It plays roles in the regulation of several cellular processes through its binding capabilities acting as a modular unit that mediates various cellular interactions.
Biological function summary
Several cellular activities rely on TRANK1 due to its ability to participate in multiprotein complexes. It regulates transcription and cell cycle processes impacting how cells grow and respond to environmental signals. By modulating the activity of other proteins TRANK1 enables cells to maintain homeostasis and adapt to changes. These actions suggest a role in normal brain functions where regulatory precision is needed.
Pathways
The TRANK1 protein interacts within the Wnt signaling and stress response pathways. These pathways involve proteins that regulate gene expression in response to extracellular signals affecting cell fate and proliferation. In the Wnt signaling pathway TRANK1 can influence components like β-catenin impacting processes like cell differentiation. It ensures proper signaling flow affecting cellular responses and maintaining healthy cellular states.
TRANK1 links to psychiatric conditions specifically bipolar disorder and schizophrenia. Research indicates aberrant expression or mutations impair normal brain functionality potentially disrupting emotion regulation and cognitive abilities. TRANK1 associates with proteins like DISC1 which are implicated in brain development and psychiatric disorders suggesting its involvement in neuronal signaling or synaptic functions. Understanding TRANK1 function in these contexts may provide insights into novel therapeutic strategies.