Abcam, ab267194, Human OASL knockout A549 cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
OASL KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2,
Disease:Carcinoma

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-OASL, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
OASL has connections to viral infections and autoimmune disorders. During infections by viruses like hepatitis C OASL contributes to the host's defense by limiting viral replication. Additionally its involvement in autoimmune conditions such as systemic lupus erythematosus demonstrates its role in immune system regulation. In these contexts OASL associates with proteins like MAVS which facilitate innate immune signaling and modulate disease mechanisms.
OASL also known as 2'-5'-oligoadenylate synthetase-like protein functions mechanically by enhancing antiviral defense mechanisms. This protein weighs approximately 58 kDa and is expressed in various cell types including immune cells such as monocytes and lymphocytes. OASL belongs to the family of interferon-stimulated genes and shares structural similarities with other 2'-5'-oligoadenylate synthetases.
Biological function summary
OASL plays a significant role in the immune system's response to viral infections. It does not form a complex but aids in the synthesis of 2'-5'-linked oligoadenylates which activate RNase L leading to the degradation of viral RNA. By doing so OASL helps to restrict viral replication and propagation within the host organism strengthening the antiviral response.
Pathways
OASL is actively involved in the interferon signaling pathway and the innate immune response pathway. It works closely with other proteins such as RIG-I-like receptors which are essential for detecting viral RNA and triggering downstream signaling processes. These interactions highlight OASL's integral function in coordinating antiviral defense strategies within these pathways.