Abcam, ab267257, Human SHPK (SHK) knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
SHPK KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 4. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 4

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SHPK, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SHK also known as Shaker-related voltage-gated potassium channel or Kv1.3 functions in the regulation of ion flow across the cell membrane. It belongs to the voltage-gated potassium channel family and has a molecular mass of approximately 57 kDa. Expression of SHK occurs mainly in the nervous system including the brain and in lymphocytes playing an important role in maintaining the cellular membrane potential and modulating action potentials.
Biological function summary
This channel is involved in repolarizing the neuronal membrane following action potentials. SHK channels contribute significantly to the excitability of neurons and T cells facilitating their activation and response. The protein does not exist in isolation but forms part of a complex with other ion channels impacting cellular excitability and immune responses. This interaction with other ion channels allows it to fine-tune the physiological responses important for cellular signaling.
Pathways
SHK participates in important neurological and immune system pathways. It is part of the neuronal signaling pathways where it affects neurotransmitter release. Additionally SHK aligns with the JAK-STAT pathway in T cells influencing their maturation and function. SHK operates alongside proteins like Kv1.1 in these pathways interacting to modulate channel conductance and overall cellular activity.
It is associated with multiple sclerosis and type 1 diabetes. SHK channel dysregulation affects the immune system contributing to aberrant immune responses seen in these autoimmune diseases. The protein interacts with other channels including Kv1.5 influencing disease progression. Targeting SHK for therapeutic intervention offers possible strategies for managing these conditions by correcting the dysregulated channel activity.