Abcam, ab267273, Human TXNRD3 knockout HEK-293T cell line

Size: 2 x 1000000Cells / vial / 1000000Cells / vial
TXNRD3 KO cell line available to order. KO validated by. Free of charge wild type control available. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 9 and 1 bp insertion in exon 9. To order both knockout and wild-type control cells: select 2 x 1000000Cells/vial. To order only knockout cells: select 1000000Cells/vial.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Form:LiquidSee storage information,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 9 and 1 bp insertion in exon 9

Product details:
We will provide viable cells that proliferate on revival.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-TXNRD3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Dry Ice, Appropriate short-term storage conditions--196°C, Appropriate long-term storage conditions--196°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
TXNRD3 also known as thioredoxin reductase 3 is an enzyme that plays a role in reducing thioredoxin proteins using NADPH as a cofactor. It typically exists as a homodimer and carries a mass of approximately 70 kDa per subunit. TXNRD3 is located in diverse tissues including testis and various other organs reflecting its versatile role in metabolism and cellular defense against oxidative stress.
Biological function summary
The enzyme functions to maintain a reduced cellular environment by reducing thioredoxin which in turn affects other cellular proteins. TXNRD3 is part of the thioredoxin system a complex that includes thioredoxin and NADPH which are essential for redox homeostasis and cellular defense mechanisms. It drives the reduction of oxidized forms of biomolecules supporting processes like DNA synthesis and repair.
Pathways
TXNRD3 significantly contributes to redox signaling and antioxidant defense. It has a pivotal position in the cellular antioxidative defense pathway by working closely with glutaredoxin and thioredoxin systems. TXNRD3 interacts with proteins such as thioredoxin helping regulate cellular redox states and protect cells from oxidative damage through these pathways. These interactions are critical for maintaining cellular functions under oxidative stress conditions.
TXNRD3 has connections with conditions involving oxidative stress and redox imbalance such as cancer. Alterations in TXNRD3 expression or activity might lead to dysregulation of redox homeostasis. In cancer the enzyme's relationship with thioredoxin and other redox-regulating proteins plays a part in tumorigenesis. Understanding TXNRD3's roles provides valuable insight into the development and progression of disease and emphasizes potential therapeutic targets for intervention.