Abcam, ab267588, Mouse Limitin ELISA Kit

Size: 1 x 96Tests
Mouse Limitin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Mouse Limitin in Mouse in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Mouse,
Assay type:Sandwich (quantitative),
Sensitivity:= 6.2 pg/mL,
Range:6.14 - 1500 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Mouse Limitin ELISA Kit is designed for the quantitative determination of Limitin in cell culture supernatants, plasma and serum samples.
Δ Note:
Mouse Limitin concentration is low in normal serum/plasma and may not be detectable in this assay.
This assay employs an antibody specific for mouse Limitin coated on a 96-well plate. Standards and samples are pipetted into the wells and Limitin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Limitin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Limitin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse BAFF R, Betacellulin (BTC), C5a, CCL6, CD48 (SLAMF2), CD6, CD80 (B7-1), Chemerin, Clusterin, Cystatin C, DAN, DLL4, EDAR, Endocan, Fetuin A, H60, IL-33 (IL-1 F11), IL-7 R alpha, Kremen-1, Lipocalin-2 (NGAL), LOX-1, Lungkine (CXCL15), Marapsin, MBL-2, Meteorin, Nope, NOV (CCN3), Osteoactivin (GPNMB), OX40 Ligand, P-Cadherin, Periostin, PlGF 2, Progranulin, Prostasin, Renin 1, Testican 3, TIM-1 (KIM-1), TRAIL (TNFSF10), Tryptase epsilon.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C