Abcam, ab267599, Human IL36RN ELISA kit

Size: 1 x 96Tests
Human IL36RN ELISA kit is a Sandwich (quantitative) ELISA for the measurement of Human IL36RN in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture supernatant, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 0.07 ng/mL,
Range:0.069 - 50 ng/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human IL36RN ELISA Kit is designed for the quantitative determination of IL36RN in cell culture supernatants, plasma and serum samples.
Δ Note:
Human IL36RN concentration is low in normal serum/plasma and may not be detectable in this assay.
This assay employs an antibody specific for human IL36RN coated on a 96-well plate. Standards and samples are pipetted into the wells and IL36RN present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL36RN antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL36RN bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 RA (IL-1 F3), IL-18 (IL-1 F4), IL-36 alpha (IL-1 F6), IL-37 (IL-1 F7), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-38 (IL-1 F10), IL-33 (IL-1 F11).

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C