Abcam, ab267602, Human GASP-1 ELISA Kit

Size: 1 x 96Tests
Human GASP-1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human GASP-1 in Human in Biofluids, Cell Culture Media samples.
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Cell culture media, Serum,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 20 pg/mL,
Range:20.48 - 5000 pg/mL,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human GASP-1 ELISA Kit is designed for the quantitative determination of GASP-1 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human GASP-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and GASP-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human GASP-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of GASP-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ACE-2, aFGF, Albumin, AMICA, Angiopoietin-4, BAFF, CA19-9, CD163, Clusterin, CRTAM, CXCL14, Cystatin C, Decorin, Dkk-3, DLL1, Fetuin A, FOLR1, Furin, GASP-2, GCSF R, HAI-2, IL-17 RB, IL-27, LAG-3, LDL R, Pepsinogen 1, RANK, RBP4, SOST, Syndecan-1, TACI, TFPI, Thrombospondin 1, TRAIL R2, TRANCE, Troponin I, uPA, VECadherin, WISP-1.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GPRASP1 also known as GASP1 stands for G-protein coupled receptor associated sorting protein 1. This protein regulates the post-endocytic sorting of G-protein coupled receptors (GPCRs). GPRASP1 interacts with various GPCRs affecting their trafficking and downregulation. Its molecular mass is approximately 125 kDa. Researchers have observed its expression in several tissues with higher levels in the brain indicating a significant role in neuronal processes.
Biological function summary
GPRASP1 plays a role in the modulation of receptor signaling by aiding in the internalization and degradation of target receptors. It functions as a part of a larger protein complex involved in receptor sorting. In the central nervous system GPRASP1 affects synaptic plasticity impacting neurological communication and receptor desensitization. This interaction highlights its regulatory influence on receptor fate after ligand binding.
Pathways
Signaling processes involve GPRASP1 particularly in the GPCR signaling pathways. GPRASP1 collaborates with proteins like beta-arrestins to mediate receptor internalization and trafficking. This protein also connects to the ubiquitination pathway by interacting with E3 ubiquitin ligases which tag receptors for degradation. These pathways demonstrate GPRASP1's pivotal position in cellular communication and receptor lifecycle management.
Researchers associate GPRASP1 with neurological conditions. Alterations in GPRASP1 function can contribute to disorders like schizophrenia and depression. GPRASP1 interaction with dopamine receptors important in mood regulation and psychotic disorders highlights this connection. These interactions have implications for pharmacological interventions aiming to modulate GPCR sensitivity and targeting in disease contexts.